C25 Phytochemical characterization and proposal of a cardiotonic mechanism of action of the methanolic extract of Caloncoba glauca (Flacourtiaceae) roots on isolated frog's heart

Auteurs

  • M. H. J Nko’o
  • Maniepi J. S Foumane
  • Ndongo M Nyangono
  • Lobe V Soppo
  • Mvondo J. M Mbole
  • Mekoulou F. C Benga
  • Touko A Djiabe
  • Ekoudi M Emanda
  • Ngombi A. P Minyem
  • Maniben B. P Ndzie
  • Fouda P Obono
  • F. A Toze
  • D. E Tsala
  • Mpondo E. A Mpondo
  • J.C Ndom
  • Nnanga Nga

DOI :

https://doi.org/10.57220/jatpb.v2i3.113

Mots-clés :

Flacourtiaceae - Caloncoba glauca – Friedelane – Cardiotonic

Résumé

Introduction: Africans have been using traditional medicine for generations. One of the faced difficulties in standardizing therapeutic protocols using phytomedicines is the elucidation of the pharmacological mechanism of action, which is essential to better understand how these therapies deal with the body. The aim of this work was to suggest a cardiotonic mechanism of action of Caloncoba glauca (Flacourtiaceae) phytocomponents on isolated frog’s heart.

Methodology: Structural characterization of the isolated compounds was made possible through the interpretation of their NMR (13C and 1H) spectral data and for the known compounds by comparing the data with those described in literature. Cardiotonic activity on isolated frog’s heart had been evaluated using kymograph through four cardiac receptors using propranolol, amlodipine, digoxine then atropine as referential drugs.

Results: The 22 obtained compounds were as follow: Triterpenes (09) - Sterols (06) - Flavonoids (03)

- Coumarins (02) - Fatty acids (02). The cardiotonic activity showed that the methanolic extract of the roots of C. glauca (Flacourtiaceae) and 3α-ethanoyloxy-30-nor-(D:A)-friedo-olean-20(29)-ène-27,22α- lactone increase cardiac parameters through the stimulation of beta-adrenergic pathway and calcium channel. Finally, molecular interactions through computer simulations showed that, for 3α-ethanoyloxy- 30-nor-(D:A)-friedo-olean-20(29)-ène-27,22α-lactone, hydrogen bonds are established between its carbonyls, arginine and glycine.

Conclusion: At the end of this work, total of 22 compounds belonging to 5 chemical sub-families were isolated, and the cardiotonic activity assay showed that the methanolic extract of the roots of C. glauca(Flacourtiaceae) and 3α-ethanoyloxy-30-nor-(D:A)-friedo-olean-20(29)-ène-27,22α-lactone increase cardiac parameters probably through the stimulation of beta-adrenergic pathway and calcium channel.

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Publiée

2023-12-20

Comment citer

Nko’o, . M. H. J., Foumane, . M. J. S., Nyangono , N. M., Soppo , L. V., Mbole, . M. J. M., Benga, . M. F. C., Djiabe , T. A., Emanda, . E. M., Minyem, . N. A. P., Ndzie, . M. B. P., Obono, . F. P., Toze, . F. A., Tsala, . D. E., Mpondo, . M. E. A., Ndom, . J., & Nga, N. . (2023). C25 Phytochemical characterization and proposal of a cardiotonic mechanism of action of the methanolic extract of Caloncoba glauca (Flacourtiaceae) roots on isolated frog’s heart. Journal Africain De Technologie Pharmaceutique Et Biopharmacie (JATPB), 2(3). https://doi.org/10.57220/jatpb.v2i3.113

Numéro

Vol. 2 No 3 (2023): Édition Spéciale - Livre des résumés

Rubrique

Résumé

Catégories

Licence

(c) Tous droits réservés M. H. J Nko’o, Maniepi J. S Foumane, Ndongo M Nyangono , Lobe V Soppo , Mvondo J. M Mbole, Mekoulou F. C Benga, Touko A Djiabe , Ekoudi M Emanda, Ngombi A. P Minyem, Maniben B. P Ndzie, Fouda P Obono, F. A Toze, D. E Tsala, Mpondo E. A Mpondo, J.C Ndom, Nnanga Nga 2023

Creative Commons License

Ce travail est disponible sous la licence Creative Commons Attribution 4.0 International .

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